Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae

KD Hampton, RA Stallings… - Journal of Clinical …, 1979 - Am Soc Microbiol
KD Hampton, RA Stallings, BL Wasilauskas
Journal of Clinical Microbiology, 1979Am Soc Microbiol
The Phadebact gonococcus test was performed on 314 bacterial isolates to compare the
coagglutination technique with the Minitek system for the confirmation of Neisseria
gonorrhoeae. Direct testing, using colonies of N. gonorrhoeae mixed with the Phadebact
gonococcus test reagents, produced noninterpretable results in many cases. However, an
alternative method, described by the manufacturer, of heating several colonies in distilled
water for 20 min before testing alleviated most interpretation difficulties. Complete …
The Phadebact gonococcus test was performed on 314 bacterial isolates to compare the coagglutination technique with the Minitek system for the confirmation of Neisseria gonorrhoeae. Direct testing, using colonies of N. gonorrhoeae mixed with the Phadebact gonococcus test reagents, produced noninterpretable results in many cases. However, an alternative method, described by the manufacturer, of heating several colonies in distilled water for 20 min before testing alleviated most interpretation difficulties. Complete agreement was achieved with both the Phadebact and Minitek tests for all 236 isolates of N. gonorrhoeae. Of the 78 nongonococcal isolates tested with Phadebact, two isolates gave false-positive results, whereas one isolate was reported as noninterpretable. The Phadebact gonococcus test appears to be a simple and rapid alternative to confirmatory biochemical procedures for N. gonorrhoeae.
American Society for Microbiology
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